Efeitos do Resveratrol na viabilidade, proliferação e apoptose de células B16F10 de Melanoma cutâneo murino: estudos in vitro

Abstract

Resveratrol (Resv), a natural polyphenolic nutrient, acts as a negative modulator of molecular pathways related to tumor progress, including control of the cell cycle and programmed cell death of cancer cells. Rev is a low toxicity adjustment in normal cells, used adjuvantly during antineoplastic treatment and it seems to promote a reduction in the resistance of cancer cells to drugs used in experimental studies. Cutaneous melanoma (CM) is a cancer that originates from skin melanocytes. This type of cancer represents a major public health problem in many human populations around the world. Cynical studies demonstrate that this cancer is quite resistant to conventional antineoplastic treatment due to its progression, notably the ability to originate metastases in the early stages of lesion development, and resistance to current chemotherapy treatments. In this context, this study focused on investigate the in vitro effects of Resv on cell viability, proliferation and programmed cell death in B16F10 murine CM cells. For this, the cells were cultured and treated with different concentrations of Resv, in order to investigate the viability via MTT of the assay. The effect of Resv (25, 50, and 100 µg/mL) on the apoptosis of B16F10 cells was investigated by carrying out the method of incorporating propidium iodide / acridine orange, quantifying Bax and Caspase-3 gene expression via real-time reaction quantitative polymerase chain (RT q-PCR) and DNA fragmentation by flow cytometry. The cell cycle was evaluated by flow cytometry using DNA staining with propidium iodide. The results demonstrated that Resv reduced cell viability in a concentration-dependent manner and confirmed the potential for apoptosis inducing this compound in murine CM-B16F10 cells. In addition, the results of the cell cycle arrest showed that cells exposed to Resv in the lowest evaluated lows (25 and 50 µg/mL) exhibited an increase in the cell population in the G2 / M phase, while the concentration of 100 μg/mL showed a proportion of cells in each phase compatible with the control group, however, in cell death tests, it was a concentration that demonstrated greater apoptotic activity. Thus, it is concluded that Resv exhibits antineoplastic effects in murine MC B16F10 cells via induction of cell proliferation inhibition, promotion of cell death by apoptosis and cell cycle block in these cells.