O estado da arte e a ação da flavona crisina em linhagens do Carcinoma de Células Escamosas Oral

Abstract

The objective of this work was to evaluate the state of the art of research involving the flavone chrysin and oral squamous cell carcinoma (OSCC) through an integrative literature review and evaluate the action of the same flavone in strains of OSCC. For this, articles were searched in PubMed, Web Of Science, Wiley Online Library, Scopus and gray literature databases. An integrative literature review instrument was used to extract data from the articles. The SciRAP tool assessed the reliability and relevance of the data. PRISMA was used to verify some essential points in reviews. Cytotoxicity, migration, proliferation, cell death, detection of reactive oxygen species (ROS) tests in OSCC cell lines SCC9 and CAL-27 and in the normal keratinocyte line HaCaT, in addition to molecular docking between chrysin and HIF-1a were performed. As for the results of the review, six articles were included at the end to be evaluated. They evaluated the action of chrysin in vivo and in vitro on oral carcinoma cell lines SCC9, KB and CAL-27. Some of the parameters evaluated were absorption, cell cycle regulation, oral bioavailability (in vivo), mitochondrial dysfunction and protein expression. The main methods used were the MTT assay, western blot and flow cytometry, in addition to chemical carcinogenic induction in animals. Chrysin induced apoptosis, regulated the cell cycle, altered the membrane potential and induced the activation of caspases involved in the apoptotic process of cells, in addition to affecting the components of the PI3K/AKT pathway. A weakness of chrysin refers to it’s oral bioavailability. Some studies used an inadequate cell lineage and others did not adequately describe the methods, which affects their reliability. Regarding the experimental work, chrysin reduced cell proliferation and migration in the two cancer strains within 24 hours, but not in HaCaT. In 48h, there was a reduction in all strains. Cell death and dose-dependent increase in ROS production were found in SCC9 and CAL-27 strains, but not in HaCaT. Regarding the protein-ligand interaction, HIF-1a bound to chrysin, establishing several chemical bonds. This could be a possible way used by chrysin to perform the effects on the tested cancer strains. Thus, it can be concluded that chrysin has potential for the treatment of OSCC and can act in different ways to exert its antitumor effects. Encapsulation increases its bioavailability and allows for combination with traditional OSCC chemotherapeutics.