Efeito dos transtornos de ansiedade no desenvolvimento do Carcinoma de Células Escamosas de Cabeça e Pescoço e avaliação do efeito antineoplásico do extrato de semente de Persea americana Mill nas linhagens SCC9, CAL 27 e Hacat

Abstract

More than 650,000 head and neck cancers are diagnosed each year worldwide. More than 90% of head and neck cancers are squamous cell carcinomas. Some of the main risk factors for this neoplasm include tobacco use and alcohol consumption. Radiation is one of the main treatment modalities for the management of head and neck squamous cell carcinoma and treatment decisions are determined taking into account factors such as primary site, stage, surgical accessibility, among other factors.Our first study aimed to assess whether anxiety disorder (AD) is related to the development of head and neck squamous cell carcinoma (HNSCC). This study is a combination of a baseline animal study and a retrospective case and control study of patients with and without cancer. As a result, we found that stress reduced the locomotor activity of animals in the stress and stress + 4NqO group (p < 0.0001). Stress showed no influence on the progression of cancer in mice. Likewise, the case group showed no differences in anxiety scores in relation to the control. In addition, no association was observed between the staging of the HNSCC and the anxiety scores in patients. In conclusion, our in vivo findings in humans and animals showed that there is no relationship between AD and HNSCC. The second study is a pilot study of the effect of a butanolic extract from the seed of Persea Americana which showed us that it has strong potential to become a more robust and complete study. The pilot study aimed to evaluate the effect of a herbal treatment for an oral neoplasm. Preliminary in vitro data show that the butanolic extract can be an adjuvant treatment to commonly performed treatments. We emphasize that it is still necessary to carry out more in vitro analyzes and an in vivo study such as the formation of reactive oxygen species and cell death by the ethidium bromide and acridime orange method to understand the mechanism that the extract acts on neoplastic cells. We believe that we may have more promising data for the treatment of oral cancer.