Eficácia da resina e do extrato etanólico de folhas de Protium spruceanum (BENT) engl. em associação com cipermetrina no controle de Rhipicephalus (Boophilus) microplus (CANESTRINI, 1887)

Abstract

Rhipicephalus microplus is one of the most important ectoparasites worldwide. This tick has an economic impact on ruminant production by transmitting harmful diseases that impair their weight gain and milk production. Conventional tick control depends mainly on the use of chemical acaricides, however, their intensive use has led to the rapid emergence of resistant tick populations. Biological products are presented as a promising measure to control the population of this ectoparasite. This study aimed to investigate the in vitro acaricidal effect of the ethanolic extract of leaves and in natura resin of Protium spruceanum (Benth.) in binary combination with a synthetic acaricide product on R. microplus. The technique used was the package of larvae (TPL), where associations between Ethanol Extract (10.19 to 101, 92 mg ml-1) or vegetable resin (5.01 to 50.18 mg ml-1) and the cypermethrin-based acaricide (15 to 150 mg ml-1) in different concentrations. In the study, the ethanolic extract or resin combined with cypermethrin presented 90% mortality on the larvae in the strains of São João da Ponte and Santa Rita, while the treatments did not reach satisfactory efficacy in relation to the engorged females. In this study, ethanol extract was more effective than in natura resin against ticks, representing an alternative active agent for the integrated control of R. microplus.