Investigação de marcadores de metabolismo glicolítico e de adesão celular no carcinoma de células escamosas bucal

Abstract

The present study aimed to test two hypotheses under the impact of genetic components in squamous cell carcinoma of the mouth (CCEB). The first hypothesis was to analyze if the hypoxic environment induces methylation of E-cadherin, thus reducing its expression in CCEB. Samples of potentially cancelable lesions (n = 18) and normal oral mucosa (n = 15) were submitted to methylation profile analysis (MS-PCR). In addition, immortalized cell lines (SCC9 and HaCat) were submitted to hypoxic treatment, followed by analysis of the methylation profile (MS-PCR) and mRNA expression (qRT-PCR) of E-cadherin. Methylation is not a distinct event between the groups of CCEB lesions and a potentially cancelable lesion. However, the analysis of E-cadherin mRNA expression in the immortalized cell lines showed a significant decrease in the expression of this gene under hypoxic conditions when compared to the control group, regardless of the methylation profile. The second hypothesis was to verify if the effect of immediate radiation and 24 hours increases genes related to glycolytic metabolism. For this purpose, blood samples from eight patients with OSCC were used and seven blood samples from control patients were submitted to analysis of PDH mRNA expression. In addition, the immortalized cell line SCC9 were subjected to hypoxic and radiation treatment, followed by analysis of mRNA expression (qRT-PCR) of LHD / PDH. Radiation under hypoxia increases PDH levels and, consequently, increases the migration capacity of neoplastic cells.