Avaliação dos efeitos do resveratrol e enalapril sobre metabolismo glicídico e lipídico de camundongos: marcadores lipogênicos do tecido adiposo branco

Abstract

Lipogenesis is a process that involves the synthesis of fatty acids using glucose as primary substrate. Resveratrol has become the target of further studies due to its ability to mimic the calorie restriction effects. Studies have shown that the ACE inhibitors may reduce insulin resistance in the liver and adipocytes by controlling serum glucose and free fatty acids levels. Enalapril has also been the target of studies aimed to elucidate its mechanisms in the metabolic regulation of carbohydrates and lipids. The objective of the present study was to evaluate the effects of oral treatment with resveratrol and enalapril on lipid and glucose profile in mice fed normolipid diet. Male Swiss mice were divided into four groups and treated for eight weeks: Standard diet + ad libitum (G1), Standard diet + Resveratrol (G2), Standard diet + Enalapril (G3), Standard diet + Resveratrol + Enalapril (G4), being the resveratrol administered with food and enalapril water. Body weight, lipid profile, glycemic parameters, adipocyte area and histomorphology of epididymal adipose tissue and weight of epididymal, mesenteric and retroperitoneal adipose tissues weight were assessed. The FAS, ACC, PPARγ and SRBP-1c mRNA expression levels were assessed by RT-PCR. The main results evidenced insulin sensitivity and glucose tolerance improvements in the group G2 as compared to G1. Similar results were also found for the fasting glucose levels. Decreased serum triglycerides levels were observed in animals treated with resveratrol and enalapril (G4), along with a decrease in the epididymal adipose tissue weight. A decrease in the FAS, ACC and PPARγ mRNA expression levels in the G4 animals as compared to G1 group. In conclusion, it was observed that the association between resveratrol and enalapril improved the glycaemic and lipid profile and the expression of a few lipogenic genes.